We demonstrated that microfluidic device can be used to culture living cells and enables high resolution hilo imaging of microtubules without perturbing their dynamics. Highly inclined and laminated optical sheet hilo microscopy significantly improves the signal to background ratio by increasing the image intensity and decreasing the background intensity. Other optical sectioning schemes like total internal reflection microscopy tirf or highly inclined laminated optical sheet microscopy hilo already became very successful in this field but because of their limited penetration depth they are not suitable for single molecule tracking in extended 3d samples.
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